Analysis Note

ControlBrainCultured neurons

Application

Research CategoryNeuroscience

Immunohistochemistry:
5-10 µg/mL on frozen or paraffin embedded tissue. See protocols for details.

Western Blot:
1:1000. 7.5%-10% SDS-PAGE

Optimal working dilutions and protocols must be determined by end user.

Anti-Neurofilament 160 kDa Antibody, clone NN18 is an antibody against Neurofilament 160 kDa for use in IH, IH(P) & WB.

Research Sub CategoryNeurofilament & Neuron MetabolismNeuronal & Glial Markers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

Immunogen

Purified neurofilament polypeptides.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: CBL214

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Neurofilament on 10 µg of PC12 lysates.

Specificity

The antibody reacts with neurofilament 160 kD (Debus, E., 1982; Shaw, G., 1984).

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Do not freeze.

Target description

160 kDa